Allelic variation distribution of vernalization and photoperiod genes in wheat from Central Asian countries

[Objective]Identification of vernalization and photoperiod genes in wheat from Central Asian countries provides theoretical foundation for introduction and reasonable selection of parental materials.[Method]65 wheat germplasm were detected by molecular markers of vernalization genes Vrn-A1,Vrn-B1,Vrn-D1 and Vrn-B3,and photoperiod gene Ppd-A1,Ppd-B1 and Ppd-D1.[Result]The results showed that distribution frequencies of Vrn-D1,Vrn-A1,Vrn-B1,Vrn-B3b and vrn-A1/vrn-B1/vrn-D1/vrn-B3 were 12.31%,6.15%,44.62%,1.54%and 46.2%,respectively.Four polymorphisms were identified at Ppd-D1 locus,forming 10 haplotypes(Ppd-D1_Hapl_I-Ppd-D1_Hapl_X).Among them,the haplotype Ppd-D1_Hapl-I was dominant,with the distribution frequency of 56.9%.At Ppd-B1 site,55 wheat germplasm were detected for the photoperiod sensitive Ppd-B1b and 10 wheat germplasm did not have PCR amplification bands.All germplasm at Ppd-A1 site were photoperiod sensitive Ppd-A1b.[Conclusion]The vernalization and photoperiod gene types and their distribution of wheat from Central Asian countries were clarified to promote the effective use of foreign resources in wheat breeding in China.