基于CRISPR/Cas9介导抗旱耐盐PagHyPRP1基因编辑杨树获得及抗性评价1
CRISPR/Cas9-mediated mutations of PagHyPRP1 improves drought and salt tolerance in poplar2
[目的]由于干旱、半干旱和盐碱地区生态环境恶劣,植树造林和植被恢复困难,因此迫切需要创造抗逆林木新品种实现困难立地的栽培利用。[方法]本研究利用CRISPR/Cas9系统对模式树种 84K杨(Populus alba×P.glandulosa)根部主要表达的盐和干旱胁迫响应负调控因子富含脯氨酸蛋白等位基因PagHyPRP1(PagHyPRP1A和PagHyPRP1B)进行多位点基因组精准编辑。利用PCR产物测序和单克测序技术对编辑植株靶点进行编辑…查看全部>>
[Objective]Due to the adverse ecological environment in arid,semi-arid and saline-alkali areas,afforestation and vegetation restoration are difficult.It is urgent to create new varieties of stress-resistant trees to realize the cultivation and utilization of difficult sites.[Method]The CRISPR/Cas9 system was used for multilocus genome editing of PagHyPRP1(including alleles PagHyPRP1A and PagHyPRP1B),a negative regulator of salt and drought stress response in…查看全部>>
张伟溪;张腾倩;丁昌俊;胡赞民;范成明;张静;李政宏;沈乐;张冰玉;刘桂丰;苏晓华;
CRISPR/Cas9 杨树 HyPRP1 干旱胁迫 盐胁迫 ROS 离子流
CRISPR/Cas9 poplar HyPRP1 salt stress drought stress ROS ion flux
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