Heterozygosity analysis of spontaneous 2n female gametes and centromere mapping of the diploid Hevea brasiliensis based on full-sib triploid populations
Heterozygosity analysis of spontaneous 2n female gametes and centromere mapping of the diploid Hevea brasiliensis based on full-sib triploid populations
[Objective]The evaluation of maternal heterozygosity restitution(MHR)is vital in identifying the mechanism of 2n gametogenesis and assessing the utilization value of 2n gametes.[Method]In this study,three different full-sib triploid populations were employed to evaluate the MHR of 2n female gametes of rubber clone GT1 and confirm their genetic derivation.[Result]The results indicated that the 2n female gametes of GT1 are derived from second division restitution(SDR)and transmit more than half of the parental heterozygosity.In addition,low recombination frequency markers were developed,and four candidate physical centromeres of rubber were located for the first time.[Conclusion]The confirmation of 2n female gametes of rubber clone GT1 are derived from SDR provides insights into the molecular mechanisms of 2n gametogenesis.Additionally,the identified centromere location will aid in the development of centromeric markers for the rapid identification of 2n gametogenesis mechanism.
Yuan-yuan Zhang;Hong-kun Li;Xiao Huang;Yu-jiao Yuan;Xiao-fei Zhang;Xin-sheng Gao;Xiang-jun Wang;Ming-ming Wei;Hua-sun Huang;Weiguo Li;
Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Dehong Institute of Tropical Agricultural Sciences of Yunnan Province,Ruili,Yunnan,678600,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;College of Tropical Crops,Yunnan Agricultural University,Puer,Yunnan,665099,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,P.R.China,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Centre for Rubber Breeding,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China##State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;
2n female gamete SDR centromere location full-sib population Hevea brasiliensis
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