顽拗性种子板栗脱水过程中蛋白质组分析

Proteome Analysis During Dehydration of Recalcitrant Seed Castanea mollissima Blume Abstract:[Objective]In order to investigate proteome and germinability in response to the dehydration of recalcitrant seed Castanea mollissima.[Method]Mature seeds of C.mollissima were collected from a chestnut garden in Jinan city,Shandong province,China.the proteome and germination were measured in embryonic axes at different moisture contents.[Result]The results showed that the germination rate rapidly decreased when the embryo moisture content decreased to 20%or below.The isobaric tags for relative and absolute quantitation(iTRAQ)-based proteome analysis identified 5201 trusted proteins,the 20%moisture content(MC)group had the most differentially accumulated proteins(DAPs)and down-regulated DAPs,while the 10%MC group had the most up-regulated DAPs.Bioinformatic analysis showed that there was a significant increase in DAPs associated with the antioxidant system and metabolic system at 30%MC.At 20%MC,the significant DAPs were mainly associated with ribosome and RNA in the endoplasmic reticulum.When the embryonic axis was dehydrated to 10%MC,the DAPs of"structural molecule activity"accumulated significantly.The DAPs can be used for screening natural populations of C.mollissima for dehydration-tolerant germplasm resources.[Conclusion]The seeds of C.mollissima are recalcitrant,and an MC of 20%or less had a serious impact on embryonic axis germination during rapid desiccation.The complex interactions of 912 DAPs,particularly 563 unique DAPs,need further investigation and experimental validation.The DAPs can be used to screen natural populations of C.mollissima,with the aim of discovering dehydration-tolerant germplasm resources.