Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii×nigra
Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii×nigra
Improving survival rate of afforested species is essential for forest production in saline-alkali areas.Transgenic hybrid poplars could provide potential genetic materials for adaptation to the semi-arid areas.We investigated the growth stability and adaptability of 9-year-old TaLEA transgenic lines of Populus simonii×nigra,as well as position of T-DNA insertion and stability of exogenous genes.Transgenic poplar field trials were carried out using a multi-site joint analysis.Molecular characteristics and the T-DNA insertion sites were identified by genome resequencing.The XL-9,XL-7 and XL-13 preferred lines consistently showed the highest and most stable growth across three testing sites.The exogenous gene was integrated into the genome chromosome and was expressed normally in XL-1,XL-7 and XL-9 lines after 9 years.XL-9,XL-7 and XL-13 are the preferred lines for subsequent production tests.Transgenic hybrid poplar lines can be selected for adaptability in 2 years after field experiments.The results of this study will provide further guidance for testing of transgenic trees for future forest production.
Yang Wang;Yunli Yang;Fusen Wang;Guangyu Wang;Chu Wang Wei Wang;Kun Chen;Chenrui Gu;Qibin Yu;Jing Jiang;
State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;Heilongjiang Academy of Forestry(Qiqihar Branch),Qiqihar,China;Heilongjiang Jiamao Garden Construction Co.,Ltd.Daqing,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;University of Florida,Gainesville,FL,USA;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,China;
1314-1314 / 1
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