银杏褐化与非褐化愈伤组织的转录组分析
Transcriptome Analysis of Browning and Non-browning Callus of Ginkgo biloba
[目的]本研究利用环境扫描电子显微镜和高通量测序技术对银杏愈伤组织进行细胞结构观察与转录组测序,以探索愈伤组织的褐化机理。[方法]利用环境扫描电子显微镜对银杏褐化和非褐化的愈伤组织进行细胞结构观察,并对银杏褐化和非褐化的愈伤组织进行转录组测序,利用DESeq2筛选差异表达基因,借助GO、KEGG等生物信息学分析手段探索基因功能。[结果]扫描电镜显示非褐变的愈伤组织细胞排列均匀,褐变愈伤组织的细胞表现出紊乱,排列松散无序。转录组测序共发现有 275…查看全部>>
[Objective]In this study,environmental scanning electron microscopy and high-throughput sequencing techn ology were used to observe the cell structure of Ginkgo biloba callus and sequence the transcriptome to explore the brow ning mechanism of callus.[Method]Using environmental scanning electron microscope to observe the cell structure of the browned and non-browning callus of Ginkgo biloba,and sequence the transcriptome of the browned and non-browning ca ll…查看全部>>
徐奇;杨晓明;汪贵斌;曹福亮;
Ginkgo biloba Callus Browning and non-browning Transcriptome Differentially expressed gene
2081-2090 / 10
评论