In vitro culture of Cyclocarya paliurus(Batal.)Iljinskaja through direc t adventitious shoot regeneration
In vitro culture of Cyclocarya paliurus(Batal.)Iljinskaja through direc t adventitious shoot regeneration
Cyclocarya paliurus(Batal.)Iljinskaja is a special species widely used in medicinal health,native to China.In this study,an efficient tissue culture system for C.paliurus has been established from nodal stem segments as explants.For adventitious shoot bud induction,MS medium combined with 6.0 6-benz ylaminopurine(BAP)mg·L-1 was the best treatment,showing maximum response for induction frequency of 96.67±3.85.Different concentrations and combinations of plant growth regulators(IBA and TIBA)t ested for shoot multiplication,maximum number of shoots(2.13±0.50)regenerated on MS medium con taining 1.5 mg·L-1 indole-3-butyric acid(IBA)and 0.02 mg·L-1triiodobenzoic acid(TIBA).The regenerate d shoot buds were elongated on MS medium supplemented with 1.5 mg·L-1 IBA(4.82±0.09).Microsh oots were rooted successfully with highest rooting response(67.59%)on WPM medium containing 2.0 m g·L-1 IBA.The present in vitro regeneration protocol could be used for large-scale propagation and conse rvation of C.paliurus.
Yuan Cheng;Yang Zhang;Jinping Cheng;Xiangxiang Fu;
College of Forestry,Nanjing Forestry University,Nanjing 210037,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,China;
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