黑松下胚轴和针叶胚性愈伤组织的诱导
Induction of embryonic callus from hypocotyl and needle of Pinus thunbergii Parl.
[目的]以黑松下胚轴为外植体,进行体细胞胚性愈伤组织的诱导研究。找出影响愈伤组织诱导的关键因素,并探讨诱导过程中培养基种类、生长素 2,4-D和细胞分裂素 6-BA浓度对愈伤组织的影响,为黑松营养器官体细胞胚胎发生体系的建立奠定基础。[方法]采用三因素四水平正交试验设计,分别以MS、WPM、B5、DCR作为基本培养基,添加不同浓度的 2,4-D(0.5 mg·L-1,1.5 mg·L-1,2.0 mg·L-1,2.5 mg·L-1)和 6-BA(…查看全部>>
[Objective]To lay the foundation for the establishment of somatic embryogenesis system in the vegetative organs of Pinus thunbergii Parl,the induction of somatic embryogenic callus(EC)was studied using hypocotyls as explants to find out the key factors affecting EC induction,and investigate the effects of medium type,concentration of auxin(2,4-D)and cytokinin(6-BA)on the EC induction.[Method]Orthogonal experiment design with three factors and four leve…查看全部>>
代静;郭辰昊;刘静;孟鹏;
Somatic embryogenesis Embryogenic callus Pinus thunbergii Parl Hypocotyl Needle
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