杨小舟蛾性信息素结合蛋白基因鉴定及功能研究
Identification and Functional Analysis of pheromone binding proteins in Micromelalopha sieversi(Lepidoptera:Notodontidae)
[目的]鉴定杨小舟蛾性信息素结合蛋白基因PBPs,明确杨小舟蛾PBPs对其性信息素活性成分的结合特性,为杨小舟蛾性信息素识别机制研究奠定基础,也可为利用化学生态学手段进行杨小舟蛾综合防控提供分子靶标。[方法]基于触角转录组测序,挖掘杨小舟蛾嗅觉相关基因;通过生物信息学和分子生物学手段对各类嗅觉相关基因进行序列分析和构建系统发育树,探究杨小舟蛾与其他鳞翅目昆虫化感基因的进化关系;采用原核表达和荧光竞争结合法研究PBPs重组蛋白与杨小舟蛾信息素活性成…查看全部>>
[Objective]This study aims to identify olfactory genes of M.sieversi and the binding properties of pheromone-binding proteins to the sex pheromone active components of M.sieversi which could lay a foundation for the further study of its sex pheromone recognition mechanism,and also provide molecular targets for the comprehensive control of the pest by chemical ecological methods.[Method]The olfactory genes were identified based on antennal transcriptome seque…查看全部>>
宋靖康;陈鑫雯;顾天滋;
Micromelalopha sieversi olfactory genes phylogenetic analysis pheromone binding protein.
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