普通油茶开花与授粉期转录组测序及相关基因差异表达分析

In order to understand the Transcriptome characteristics of common Camellia oleifera during flowering and pollination,and to find the differentially expressed genes related to the reproductive process of common Camellia oleifera during this period,it provides a research basis for the molecular mechanism of common Camellia oleifera reproduction.High throughput Transcriptome sequencing was used to sequence the flowers of the common Camellia oleifera Xianglin 210#4 periods(flowers not blooming,2-3 days of pollination,7 days and 20 days of pollination).The results showed that 21.57Gb Clean reads were obtained after removing low-quality data.When the threshold value was log2(multiple change)>1 or log2(multiple change)<-1,85134 Unigenes(46872 up-regulated and 38262 down-regulated)were detected.Based on six databases including GO,KEGG,Pfram,SwissProt,eggNOG,and NR,similarity comparisons were conducted,and 33290,26896,29515,26821,38129,and 40433 genes were annotated,respectively.The differentiation analysis of GO function of different genes shows that genes are largely enriched in biological processes,with 71367 different GO functions,mainly including biological processes,DNA Transcriptional regulation,DNA transcription,protein phosphorylation,protein phosphorylation,redox process,and defense response;In KEGG analysis,there were 404 and 313 differential genes in Plant hormone signal transduction,starch and sucrose metabolism enrichment,respectively.The expression level of differentially expressed genes is highest in Camellia oleifera stigma FR1,and the differentially annotated genes in the two pathways are SPL8,AHP1,SERK2,SAUR67,CXE16,SAUR21,EIN3,TGAL4,ARR6,BGLU40,SBEI,TRE1,Os10g0521000,and exgA,respectively.