雌雄异型异熟胡桃楸性别分化关键miRNA的鉴定和分析
Identification and analysis of key miRNA for sex differentiation in hermaphroditic Juglans mandshurica Maxim
胡桃楸是雌雄同株异型异熟植物,分为雌先型和雄先型两种类型,其在东北地区是良好的经济树种和生态树种。miRNA是一类内源性真核非编码RNA,在植物体内对其靶基因有沉默作用。由 miRNA介导胡桃楸性别分化和雌雄异型异熟机制尚不清楚。本研究利用高通量测序技术对胡桃楸雌雄先型的雌雄花芽进行MicroRNA测序分析,构建了12 个sRNA文库,共鉴定了310个已知的和278个未知的miRNA,对miRNA的长度、家族等信息进行统计。使用Deseq软件包筛…查看全部>>
Juglans mandshurica Maxim is a monoecious heterozygous heterozygous plant,divided into female-first and male-first types,which is a good economic and ecological tree species in Northeast China.miRNA are a class of endogenous eukaryotic non-coding RNAs,which have a silencing effect on their target genes in the plant.The mechanism of miRNA-mediated sex differentiation and dioecious heterozygosity in J.mandshurica is not clear.In this study,microRNA sequencing …查看全部>>
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