PtrDJ1C,an atypical member of the DJ-1 superfamily,is essential for early chloroplast development and lignin deposition in poplar
PtrDJ1C,an atypical member of the DJ-1 superfamily,is essential for early chloroplast development and lignin deposition in poplar
The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woody plants.This study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 superfamily.PtrDJ1C knock-out with the CRISPR/Cas9 system resulted in different albino phenotypes.Chlorophyll fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased significantly.Moreover,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant line.Furthermore,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin accumulation.We conclude that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar.
Xinwei Wanga;Chunxue Shaoa;Libo Liu;Yue Wanga;Yaqi Ana;Hui Li;Yunwei Ding;Yanping Jinga;Xiaojuan Lia;Jianwei Xiao;
Hebei North University,Zhangjiakou,075000,China;National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;Hebei North University,Zhangjiakou,075000,China;National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;Hebei North University,Zhangjiakou,075000,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China##National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China##Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;
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