蓝莓根系微生物群落在不同根系空间生态位上的互作特征

[Objective]Plant root-associated microbiomes play critical roles in promoting plant health,productivity,and tolerance to biotic/abiotic stresses.Blueberry(Vaccinium spp.)is adapted to acidic soil s,while the interactions of the root-associated microbiomes in this specific habitat remain elusive under various root microenvironments.[Method]Here we investigated the diversity and community composition of bacterial and fungal communities in various blueberry root niches(bulk soil,rhizosphere soil,and ro ot endosphere).[Result]The results showed that blueberry root niches significantly affected root-associat ed microbiome diversity and community composition compared to the host cultivars.Deterministic proces ses gradually increased along the soil-rhizosphere-root continuum in both bacterial and fungal communiti es.The co-occurrence network topological features showed that both bacterial and fungal community com plexity and intensive interactions decreased along the soil-rhizosphere-root continuum.Different compartm ent niches clearly influenced bacterial-fungal interkingdom interactions,which were significantly higher in the rhizosphere,and positive interactions gradually dominated the co-occurrence networks from the bulk soil to the endosphere.The functional predictions showed that rhizosphere bacterial and fungal communiti es may have higher cellulolysis and saprotrophy capacities,respectively.[Conclusion]Collectively,the ro ot niches not only affected microbial diversity and community composition,but also enhanced the positiv e interkingdom interactions between bacterial and fungal communities along the soil-rhizosphere-root cont inuum.This provides an essential basis for manipulating synthetic microbial communities for sustainable agriculture.