雌雄异株山桐子根际土壤微生物群落特征及功能预测分析3

[Objective]The aim of this study was to investigatediversity,structure,and functional gro up characteristics of the rhizosphere soil microbial community of dioecious Idesia polycarpa trees.[Metho d]Seven year old male and female trees were taken as materials,and samples were taken at the floweri ng stage(May)and fruit maturity stage(October)of the fruit.The characteristics of nutrient content in t he rhizosphere were analyzed.At the same time,Illumina high-throughput sequencing technology was use d to analyze the diversity of the rhizosphere of male and female trees in different periods.[Result](1)There are differences in the total nitrogen and total potassium contents in the roots of male and female trees in different periods of time.(2)The α diversity index was significantly higher during fruit ripening than during flowering,and there was no significant difference in Shannon,Simpson,and Chao1 between male and female plants during flowering.During fruit ripening,the soil Chao1 and Shannon indices of male plants were significantly higher than those of female plants.(3)The dominant bacterial phyla in th e soil of Idesia polycarpa are mainly Proteobacteria,Acidobacteria and Actinomycetes.The relative abund ance of some dominant bacterial phyla in the soil during the fruit ripening period is significantly reduce d.From the flowering period to the fruit ripening period,the dominant bacterial genera in the soil of m ale and female plants change from actinomycetes to amomorpha.(4)RDA redundancy analysis showed t hat there was a significant correlation between pH and AK and the bacterial structure in the rhizosphere of Idesia polycarpa(P<0.05).(5)PICRUSt functional prediction shows that there are a total of 6 primar y subfunctions and 47 secondary subfunctions in male and female plants at different stages,with 8 signif icantly different secondary subfunctions.[Conclusion]Our results demonstrated that during the flowering period,the dominant phylum in the soil of Idesia polycarpa is Actinobacteria,while during the fruit rip ening period,it is Proteobacteria.The number of dominant bacterial genera in the soil of female plants of Idesia polycarpa is greater than that of male plants.The functional prediction indicates that the soil b acterial ecological functions of male and female trees of Idesia polycarpa are relatively rich at different s tages,and there are significant differences in soil metabolic functions during fruit maturity and flowering stages.