氮素对棉花幼苗生长、养分分配及氮素利用效率的影响
Effects of nitrogen on cotton growth, macronutrients distribution and nitrogen use efficiency
棉花是一种重要的经济作物,其生长对氮素需求较高,但其氮素利用效率较低。本研究设置了0、0.04、0.4、1、4、8mmol•L-ICa(NO3)2共6个氮浓度,分别记为N0、N0.04、N0.4、N1、N4、N8,研究其对棉花幼苗根系和地上部形态、生物量和主要营养元素吸收及其分配、光合能力、氮吸收效率(NUpE)及氮利用效率(NUtE)的影响。结果表明:随着氮浓度的增加,棉花幼苗根系及地上部的生长、干物质质量、营养元素累积量、光合能力等均呈先增加后降低的趋势,4mmol•L-1Ca(NO3)2是棉花苗期最适宜的氮浓度。单株棉花幼苗的N、P、K、Ca的最大积累量分别为35.1mg、6.5mg、40.4 mg和16.9mg,植株体内N:P:K:Ca约为1:0.2:1.2:0.5。NUpE、NUtE与根冠比显著正相关,NUpE和NUtE还与根干物质分配比例、根平均直径、根养分分配比例等变化规律一致,它们均随氮浓度的增加而显著降低。植株N、Ca含量,地上部K含量,叶片干物质质量和地上部养分分配比例则均随氮浓度的增加而显著增加。以上结果表明,适宜氮浓度显著促进了其他养分的吸收,提高了净光合速率(P)与蒸腾速率(T),使地上部养分分配比例显著提高,从而使植株旺盛生长。适度缺氮会抑制地上部生长,提高根系养分分配比例,促进根系伸长与增殖,提高NUpE和NUtE;而极度缺氮则会降低根系数量,提高根系直径。高氮浓度显著抑制根系整体生长,并降低 NUpE 和NUtE。
Cotton is an important economic crop with high N requirements for its growth, but its nitrogen use efficiency is low. In this study, six N concentrations(0,0.04, 0.4, 1,4, 8 mmol•L-1Ca (NO3)2, named N0, N0.04,N0.4,N1, N4, N8) were set to study their effects on root and shoot morphology, biomass and macronutrients absorption and distribution, photosynthetic capacity, N uptake(NUpE)efficiency and utilization(NUtE) efficiency ofcotton seedlings. The results showed that with the increase ofN concentration, the cotton growth(include root and shoot), biomass, macronutrients accumulation, and photosynthetic capacity all showed a trend of first increasing and then decreasing, and 4 mmol•L-1Ca(NO3)2 is the most appropriate N concentration for cotton seedling growth. The maximum accumulations of N, P, K and Ca in cotton seedlings were 35.1 mg,6.5 mg,40.4 mg, and 16.9 mg per plant, respectively, and the N:P:K:Ca in plants was about 1:0.2:1.2:0.5. There was a significant positive correlation between NUpE, NUtE and the root-shoot ratio. In addition, NUpE and NUtE were also consistent with changes in the root biomass distribution proportion, root average diameter, and the root macronutrients distribution proportion, all of which decreased significantly with the increase of N concentration. The plant N content, plant Ca content, shoot K content, leaf biomass and the shoot macronutrients distribution proportion were all increased significantly with the increase of N concentration. The above results showed that the appropriate N concentration significantly promoted the absorption of macronutrients, increased the Pa and T, and significantly increased the proportion of macronutrients distribution in the shoot, thus making the plant grow vigorously. Moderate N deficiency inhibited shoot growth, increased root macronutrients distribution proportion, promoted root elongation and proliferation, and increased NUpE and NUtE, while extreme N deficiency reduced root number but increased root diameter. High N concentration significantly inhibited overall root growth and decreased NUpE and NUtE.
陈丹丹;彭杰;谢晓麒;卢丽文;杜轩;孟令浩;杨国正;
华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;华中农业大学植物科学技术学院,武汉430070;
cotton nitrogen concentration root morphology macronutrients distribution photosynthesis
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