亚热带酸雨区马尾松EM根尖真菌群落多样性分析
Fungal community on ectomycorrhizal root tips of Pinus massoniana in subtropical acid rain area
[目的]揭示亚热带酸雨区不同林型中马尾松外生菌根(EM)根尖的真菌群落多样性、结构和组成的差异,探讨EM根尖真菌群落缓解土壤酸化的机制。[方法]以重庆铁山坪林场的马尾松纯林(Pi)、马尾松-香樟混交林(Pi_Ci)以及马尾松-木荷混交林(Pi_Sc)为研究对象,采用样地调查和分子生物学相结合的方法,对不同林型中马尾松EM根尖的真菌群落进行研究。[结果](1)三种林型的马尾松EM根尖样品中共获得 3737 个真菌OTUs,隶属于 15 门 60 纲 338 科 770 属;(2)Pi纯林EM根尖真菌丰富度显著高于Pi_Sc混交林,三林型的EM根尖真菌群落多样性一致,无显著差异;(3)不同林型中马尾松EM根尖真菌群落结构组间差异显著(P＜0.05),RDA分析表明土壤中交换性Ca、Mg含量以及pH值显著影响马尾松 EM 根尖真菌群落结构;(4)Pi 纯林中主要为青霉属 Penicillium,且 Penicillium 和 EM 真菌Thelephoraceae 可能会加剧土壤酸化;Pi_Ci 混交林中有益菌占优势(Mortierella、Tausonia、EM 真菌Scleroderma和Pseudotomentella),可改善土壤养分、缓解土壤酸化;Pi_Sc混交林中的有益菌Glutinomyces可改善土壤环境,但EM真菌Meliniomyces和Tomentella可能会加剧土壤酸化。[结论]Pi_Ci混交林中的马尾松EM根尖真菌群落对缓解土壤酸化更加有益,在亚热带重庆酸雨区种植香樟可缓解土壤酸化。
[Objective]In order to reveal the differences in diversity,structure and composition of ectomycorrhiza(EM)root-tip fungal communities of Pinus massoniana in different forest types in subtropical acid rain area,and to explore the mechanism of EM root-tip fungal communities alleviating soil acidification.[Method]P.massoniana EM root-tips were collected from pure P.massoniana stands(Pi),mixed plantations with Cinnamomum camphora(Pi_Ci)or Schima superba forest(Pi_Sc),the fungal communities at the root tip of Pinus massoniana EM in different forest types was studied by means of sample survey and molecular biology.[Result](1)A total of 3737 fungi OTUs were detected from all the samples,belonging to 15 phyla,60 classes,338 families and 770 genera;(2)The richness of EM root-tip fungi in Pi was significantly higher than that in Pi_Sc,while without difference in the diversity among three forest types.(3)There were significant differences in the community structure of EM root-tip fungi of P.massoniana(P<0.05),and we also found that soil exchangeable Ca,Mg content and pH value significantly affected the community structure indicated by RDA results.(4)Penicillium was dominant in Pi,and Penicillium and the ectomycorrhizal fungus Thelephoraceae may aggravate soil acidification.Some beneficial bacteria(Mortierella,Tausonia,EM fungi Scleroderma and Pseudotomentella)were more abundant in Pi_Ci,which could improve soil nutrients and alleviate soil acidification.In Pi_Sc,more abundant Glutinomyces is beneficial for soil environment,while EM fungi Meliniomyces and Tomentella may aggravate soil acidification.[Conclusion]The EM root-tip fungi of P.massoniana in Pi_Ci contributed to alleviate soil acidification,and plantation with C.camphora in Pi pure forest could alleviate soil acidification in subtropical acid rain area.
倪秀雅;冯永霞;陈展;
中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林生态环境重点实验室,北京,100091;中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林生态环境重点实验室,北京,100091;中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林生态环境重点实验室,北京,100091;
Pinus massoniana EM root-tip fungi soil acidification fungal community
770-771 / 2
评论