新疆野苹果种子MsPYR1-like基因克隆、表达与亚细胞定位分析
Cloning,expression and subcellular localization analysis of MsPYR1-like Genes in Malus sieversii(Ledeb.)M.Roem.seeds
PYR/PYL/RCAR基因家族作为脱落酸(ABA)受体,是参与早期ABA的感知和信号传递的三个主要成分之一,种子休眠的调控主要是通过调节赤霉素(GA)和脱落酸(ABA)的代谢和信号传导途径实现的。本研究对新疆野苹果种子MsPYR1-like基因进行克隆、亚细胞定位与表达分析,为了揭示其参与新疆野苹果种子解除休眠作用机制提供理论依据。基于前期新疆野苹果种子转录组数据库,分析结果获得新疆野苹果种子PYR1-like家族基因序列,克隆得到PYR1-l…查看全部>>
The PYR/PYL/RCAR gene family,as abscisic acid(ABA)receptors,is one of the three major components involved in early ABA sensing and signaling,and the regulation of seed dormancy is mainly achieved by regulating the metabolic and signaling pathways of gibberellin(GA)and abscisic acid(ABA).In this study,the MsPYR1-like gene of Malus sieversii seeds was cloned,subcellularly localized and expressed to provide a theoretical basis for its involvement in the mechani…查看全部>>
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