不同地理唐古特白刺家系花粉形态多样性研究

In order to understand the species diversity of Nitraria tangutorum,the pollen morphology of 6 families from different geographical regions was studied.The materials in this study were collected in 2011 and planted in the same environment.The fresh pollen was freeze-dried,photographed and measured by scanning electron microscope(Sem),and the measured data of 6 families were analyzed by variance analysis,principal component analysis and cluster analysis.the pollen grains of Nitraria tangutorum existed as single grains,medium size,long globose,with three orifices,wide orifices,local connections,and the ridge surface of orifices of orifices was extremely small,the middle was large,"convex"shape increased,decorative type for the strip,brain-shaped,and did not constitute a pattern;There were significant differences in the characteristics,ridge shape and ornamentation type of the pollen,among which the variation of the pollen was the most complicated;The shape variation of the ridge surface of the germinating ditch in Ganzhou Dragon Canal of Zhangye was the largest;The decorative patterns of the old temples in Zhangye are the most abundant;Only the dragon canal in Ganzhou,Zhangye,and the Xiaobaokou Dam in minqin,Wuwei,form a"fingerprint"pattern.The coefficient of variation of pollen morphology ranged from 4.33%to 40.09%,in which the genetic stability of pollen quality traits(27.97%)was higher than that of quantitative traits(4.91%),It's mostly gene.Cluster analysis revealed that the six families were grouped into four categories,with Longqu in Zhangye and old temple in Zhangye,and Guazhou trough in Jiuquan and gold pagoda in Jiuquan.The results provide a basis for the genetic diversity and breeding of Nitraria tangutorum.