陆地棉 BAM(β-淀粉酶)基因家族的全基因组鉴定及在棉纤维发育中的功能分析

The β-amylase(BAM) is an important enzyme that catalyzes the conversion of starch to maltose in plants, and plays an important role in regulating the growth and development of various plants. So far, BAMs have been extensively studied in Arabidopsis thaliana, but the characteristics of the BAM gene family in the important economic crop, cotton have not been reported. In order to understand the structure and expression characteristics of GhBAM gene family in Gossypium hirsutum, the whole genome identification and evolutionary analysis of GhBAM were carried out, and the expression pattern of GhBAM in different fiber development stages were studied. In this study, 27 GhBAM genes of G. hirsutum were identified by genome-wide identification, and they were divided into three groups according to sequence similarity and phylogenetic relationship. The gene structure, chromosome distribution and collinearity of all GhBAM genes identified in G. hirsutum were analyzed. Further sequence alignment of the core domain of glucosyl hydrolase showed that all GhBAM proteins had Glycosyl hydrolase family 14 domain. Phylogenetic analysis showed that the BAM gene families of four cotton species and Arabidopsis thaliana were divided into three groups. This systematic analysis provides new insights into the characteristics of BAM genes in G.hirsutum, and lays the foundation for further study of the function of these genes.