青钱柳流式细胞术预测倍性方法的初步建立
Preliminary establishment of the method for predicting ploidy of Cyclocarya paliurus
[目的]本试验以不同保存状态下的青钱柳叶片为材料,探究样品与解离液的差异对流式细胞分析结果的影响,达到对青钱柳流式细胞分析方法的初步建立。试验以不同贮存方式下四倍体青钱柳幼嫩叶片为材料,探究LB01、GPB、mGb、WPB和 Tris-MgCl2解离液对鲜样(4℃)、冻样(-20、-40和-80℃)和硅胶干燥样进行流式细胞分析的适用性和贮存方式和时间的差异。[结果]对于 4℃保鲜和硅胶干燥 7 d的叶片,使用mGb解离液所出峰图效果最佳,对于-8…查看全部>>
[Objective]In this experiment,C.paliurus leaves under different storage conditions were used as materials to explore the impact of the difference between the sample and the dissociation solution on the results of flow cytometry,and to achieve the preliminary establishment of a flow cytometric analysis method for C.paliurus.The experiment used the young leaves of tetraploid C.paliurus under different storage methods as materials,and explored the effect of LB0…查看全部>>
宋子琪;卞国良;胡清华;尚旭岚;
Cyclocarya paliurus nucleus-isolation buffer Polyploid Internal standard method
802-813 / 12
评论