灵武长枣根蘖与嫁接繁殖土壤微生物的差异研究
Study on the difference of soil microorganism between Lingwu Ziziphus jujuba cv.Changzao root tiller and grafting propagation
[目的]灵武长枣因其较高的的药用价值和营养价值而备受人们青睐,同时种植面积也不断扩大,但是不同繁殖方式下灵武长枣的土壤微生物群落结构和多样性的差异尚不清楚,[方法]本文以根蘖和嫁接繁殖方式下的灵武长枣为对象,运用高通量测序技术探究这两种繁殖方式的土壤微生物群落结构和多样性以及微生物生物量的差异。[结果]结果表明:根蘖和嫁接两种繁殖方式下土壤微生物群落结构组成相似,均以Ascomycota(子囊菌门)为优势菌门,以Fusarium(镰刀菌属)和 Chaetomium(毛壳菌属)等为优势菌属。虽然 Fusarium(镰刀菌属)均为两者的优势菌属,但其在嫁接繁殖方式下所占的比例远高于根蘖繁殖,而镰刀菌能产生植物刺激素(赤霉素),可使枣树增产。由 alpha多样性分析可知嫁接繁殖下的土壤微生物群落丰富度高于根蘖繁殖,但多样性和均匀度均低于根蘖繁殖,说明嫁接土壤中微生物物种种类相对较丰富,但是物种分布不均匀。此外,土壤微生物量碳、磷含量为根蘖繁殖＞嫁接繁殖,微生物量氮含量为嫁接繁殖＞根蘖繁殖。[结论]与根蘖繁殖相比,嫁接繁殖可以提高土壤中微生物数量以及丰富度,并有效促进枣树根际微环境中碳和磷的循环,从而促进枣树植株吸收利用土壤碳、磷的能力,可有助于提高灵武长枣产量,研究结果可为科学选择灵武长枣繁殖方式提供理论依据。
[Objective]Lingwu long jujube is favored by people because of its high medicinal value and nutritional value,and the planting area is also expanding.However,the differences in soil microbial community structure and diversity of Lingwu long jujube under different breeding methods are still unclear.[Method]In this paper,Lingwu long jujube under root tillering and grafting breeding methods was used as the object,and high-throughput sequencing technology was used to explore the differences in soil microbial community structure and diversity and microbial biomass between these two breeding methods.[Result]The results showed that the composition of soil microbial community structure was similar under the two breeding methods of root tiller and grafting.Ascomycota was the dominant phylum,and Fusarium and Chaetomium were the dominant genera.Although Fusarium is the dominant genus of both,its proportion in grafting reproduction is much higher than that in root tiller reproduction,and Fusarium can produce plant hormones(gibberellin),which can increase the yield of jujube trees.The alpha diversity analysis showed that the richness of soil microbial community under grafting reproduction was higher than that under root tiller reproduction,but the diversity and evenness were lower than those under root tiller reproduction,indicating that the microbial species in the grafted soil were relatively abundant,but the species distribution was uneven.In addition,the content of soil microbial biomass carbon and phosphorus was root tiller reproduction>grafting reproduction,and the content of microbial biomass nitrogen was grafting reproduction>root tiller reproduction.[Conclusion]Compared with root tiller reproduction,grafting reproduction can increase the number and richness of microorganisms in the soil,and effectively promote the circulation of carbon and phosphorus in the rhizosphere microenvironment of jujube trees,thus promoting the ability of jujube trees to absorb and utilize soil carbon and phosphorus,which can help to improve the yield of Lingwu long jujube.The research results can provide a theoretical basis for the scientific selection of breeding methods of Lingwu long jujube.
马圆;刘佳嘉;
宁夏大学林业与草业学院;宁夏大学林业与草业学院;
Propagation by grafting Tillering propagation Soil microorganisms High-throughput sequencing Diversity
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