Effects of 6'-O-caffeoylarbutin on the activity and conformations of mushroom tyrosinase
Effects of 6'-O-caffeoylarbutin on the activity and conformations of mushroom tyrosinase
Based on a well understanding of the strongly anti-melanin activity of 6'-O-Caffeoylarbutin(CA),high performance liquid chromatography(HPLC)was performed initially to determinate the inhibitory effects of CA against the oxidation of diphenolase substrate L-dihydroxyphenlyalanine(L-DOPA)catalyzed by mushroom tyrosinase(mTyr),then the inhibition of CA on the mTyr were assayed.CA exhibited potently inhibitory effects both monophenolase activity with an IC50 value of 1.114±0.035 μM and diphenolase activity with 95.198±1.117 μM,respectively.Conformations changes of enzyme were further studied by ultraviolet-visible(UV-vis),fluorescence,and computational stimulation,results indicated that CA could be used as an inhibitor by inhibiting the release of the oxidation products and the access of L-DOPA.These results will be contributed to design and screen for mTyr inhibitors from the most abundant naturally occurring arbutin derivates.
Xiaoqin Yang;Wengan Fu;Dong Xie;Qi Wang;Kangjia Han;Sida Xie;Dong Xie;Wengan Fu;Tiantian Yuan;Guolei Zhu;Qian Jiang;Ping Zhao;
Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China##Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China##Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,China##Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650224,China;
6'-O-Caffeoylarbutin mushroom tyrosinase inhibition diphenolase inhibitory effect molecular docking
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