高表达的 BZR转录因子赋予棉花多重耐受性
Highly expressed BZR transcription factors confer multi-tolerances in cotton
油菜素内酯(Brassinosteroids,BRs)是植物体内的一种类固醇激素，在植物生长发育、光形态发生以及对非生物胁迫的响应等诸多生物学过程中发挥着重要作用。BRASSINAZOLE-RESISTANT(BZR)转录因子是BR信号通路的核心组成部分，参与了许多植物的发育过程。为了探究BZR基因在棉花中的功能，对BZR基因家族进行了生物信息学分析。通过系统发育分析，将67个BZR基因划分为3个亚家族，并通过保守基序和保守结构域分析验证了这一划分。分析了BZR基因在冷、热、盐和干旱处理下的表达水平，以预测其在非生物胁迫下的功能。BZR基因启动子分析表明，光信号可以增强BZR的转录活性。上述结果结合GhBZR蛋白相互作用网络，表明 GhBZR与PIF4的相互作用可能在棉花应对非生物胁迫中发挥重要作用。还对 GhBZR互作网络中的相关基因进行了分析讨论。这些发现为进一步了解BZR在棉花中的功能奠定了基础。
Brassinosteroids(BRs), as a type of steroid hormone in plants, play an important role in many biological processes, such as plant growth and development, light morphogenesis, and the response to abiotic stress. The BRASSINAZOLE-RESISTANT(BZR)transcription factor is a core component of the BR signaling pathway and is involved in the development of many plant species. To explore the function of BZR genes in cotton, we performed a bioinformatics analysis of the BZR gene family. Sixty-seven BZR genes were identified and divided into three subfamilies through phylogenetic analysis, and this division was verified by the analysis of conserved motifs and conserved domains. The expression level of BZR under cold, heat, salt and drought treatments were also analyzed to predict function of BZR under abiotic stress. Promoter analysis of BZR genes showed that light signaling can enhance the BZR transcriptional activity. These results combined with the GhBZR protein interaction network, demonstrated that the interaction between GhBZR and PIF4 may play an important role in the response to abiotic stress. In this study, therelated genes in the GhBZR interaction network were also discussed. These findings lay a foundation for further understanding the function of BZR in cotton.
白志刚;崔爱花;刘帅;胡启星;孙巨龙;
江西省经济作物研究所,江西九江332105;江西省经济作物研究所,江西九江332105;江西省经济作物研究所,江西九江332105;江西省经济作物研究所,江西九江332105;江西省经济作物研究所,江西九江332105;
BZR transcription factors cotton abiotic stress gene network expression analysis
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