白桦BpPIN3参与调控叶片近轴面卷曲的分子机制
Molecular mechanism of BpPIN3 involved in regulating leaf adaxial surface curling in Betula pendula
[目的]叶片是植物的营养器官之一,对植物的生长发育至关重要。PIN-FORMED(PINs)基因作为IAA运输载体,在叶片发育过程中发挥着关键作用,为了明确BpPIN3基因的功能,揭示白桦BpPIN3在叶片极性建成中的功能。[方法]本实验采用农杆菌介导法开展该基因的遗传转化研究,以BpPIN3 过表达、抑制表达株系为材料,进行叶片解剖观察、叶片栅栏组织细胞密度测定、叶片内源IAA的含量分析以及组织定位,并基于RNA-seq分析进行差异基因挖掘。[…查看全部>>
[Objective]Leaves are one of the vegetative organs of plants that are essential for plant growth and development.PIN-FORMED(PINs)gene is an indoleacetic acid(IAA)transporter that plays a critical role in leaf development.In order to clarify the function of BpPIN3 gene and reveal the function of BpPIN3 in leaf polarity formation of Betula pendula.[Method]In this study,the genetic transformation of BpPIN3 was carried out by Agrobacterium-mediated method.The Bp…查看全部>>
陈坤;渠畅;张潇月;王伟;顾宸睿;刘桂丰;于启斌;杨传平;姜静;
Betula pendula BpPIN3 IAA transport leaf Adaxial-Abaxial.
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