香榧树根系病原真菌多重荧光定量PCR检测方法的建立及初步应用
Establishment and Preliminary Application of Multiple Fluorescence Quantitative PCR Detection Method for Pathogenic Fungi in the Roots of Torreya grandis
由尖孢镰刀菌(Fusarium oxysporum)、立枯丝核菌(Rhizoctonia solani)、齐整小核菌(Sclerotium rolfsii)等土传病原菌引起香榧根腐病是香榧(Torreya grandis'Merrillii')的主要病害之一,目前常见的土壤病原菌检测法有平板培养法,但该方法存在检测周期长,无法做到菌株定量等缺点。因此本研究了建立对这三种土壤习居病原菌的快速检测及香榧病害预防的核酸检测体系,本研究基于上述三种病原菌…查看全部>>
The root rot of Torreya grandis'Merrillii'caused by soil-borne pathogens such as Fusarium oxysporum,Rhizoctonia solani,Sclerotium rolfsii,etc.One of the diseases,the common soil pathogen detection method at present is the plate culture method,but this method has the shortcomings of long detection cycle and inability to quantify the strain.Therefore,this study established a nucleic acid detection system for the rapid detection of these three soil-habiting pat…查看全部>>
寿南松;韦国春;曾燕如;戴文圣;
Torreya grandis root rot soil-borne diseases triple real-time fluorescence quantitative PCR
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