组合策略提高疏棉状嗜热丝孢菌脂肪酶在毕赤酵母中的分泌表达及其催化制备生物柴油的应用
Multiple strategies for high-efficiency expression of Thermomyces lanuginosus lipase in Pichia pastoris and production of biodiesel in solvent-free system
[目的]构建高效分泌表达疏棉状嗜热丝孢菌(Thermomyces lanuginosus)脂肪酶TLL的毕赤酵母(Pichia pastoris)工程菌,实现脂肪酶的高水平表达,以降低生物催化剂的制备成本。将获得的脂肪酶TLL用于催化制备生物柴油,考察其催化活性,为生物酶法生产生物柴油提供参考。[方法]采用多种策略来提高毕赤酵母中脂肪酶TLL的表达水平。首先对基因表达元件进行了筛选,比较了不同信号肽的分泌效率、不同启动子的启动强度和不同基因剂量对…查看全部>>
[Objective]The aim of this study was to construction a recombinant Pichia pastoris to high level secretion expression Thermomyces lanuginosus lipase to reduce the cost of biocatalyst.In order to identify the catalytical activity,the obtained TLL was applied to produce biodiesel using Jatropha curcas oil as substrate.[Method]In this study,multiple strategies were used to improve the expression level of TLL in P.pastoris.Firstly,the effects of gene expression …查看全部>>
许蕊;张瑜;李迅;王飞;
Thermomyces lanuginosus lipase Pichia pastoris non-methanol induction biodiesel.
1733-1742 / 10
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