南极假丝酵母脂肪酶B在毕赤酵母中的高效表达及其在生物柴油生产中的应用
Efficient expression of Candida antarctica lipase B in Pichia pastoris and its application in biodiesel production
[目的]本研究以毕赤酵母为宿主,构建高效表达CALB毕赤酵母工程菌,实现CALB的大量有效分泌表达,并用其催化地沟油生产生物柴油。[方法]首先,体外构建了表达CALB的质粒,该质粒为进行了CALB密码子优化后的质粒,随即选取不同的毕赤酵母为宿主,将质粒线性化后,电转化导入毕赤酵母中进行分泌表达。然后,通过查阅文献选择了7个不同类型的启动子,并在原质粒的基础上进行启动子元件的更换得到新的质粒,线性化,电转化导入毕赤酵母中进行分泌表达。在摇瓶培养后,…查看全部>>
[Objective]In this study,Pichia Pastoris was used as host to construct a CALB-expressing engineering strain to achieve a large amount of effective secreted expression of CALB,and use it to catalyze the production of biodiesel from waste oil.[Method]Firstly,a CALB-expressing plasmid was constructed in vitro,which was a plasmid optimized with CALB codon.Then different P.pastoris were selected as hosts,and the plasmid was linearized and introduced into P.pastor…查看全部>>
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