网纹马勃对中辽 1号杨生长及抗旱性影响研究

[Objective]In order to enrich the ectomycorrhizal fungi resources of poplar,promote the growth of poplar and improve its drought resistance,in this paper,ITS sequence molecules of the Lycoperdon macrofungi collected were identified,and based on the co-culture of the fungus with tissue culture seedlings of Populus×canadensis cv.'Zhongliao 1',mycorrhiza formation was observed and its effects on the poplar growth and root development were discovered.With the water control method,the physiological index changes of the potted cutting seedlings under different degrees of drought stress were analyzed to reveal the fungus effects on the poplar drought resistance.[Result]1)the macrofungi collected was Lycoperdon perlatum Pers.,2)it could form ectomycorrhiza with Populus×canadensis cv.'Zhongliao 1',and through the co-culture,compared with the control,the whole seedling biomass,root biomass,total root length,root surface area,root volume and the number of root tips were increased by 96.43%,162.07%,75.71%,108.33%,178.34%,88.81%respectively,3)under drought stress,compared with the control,the content of osmoregulatory substances such as proline and soluble sugar in seedlings inoculated with Lycoperdon perlatum Pers.was higher,the contents of peroxide product malonaldehyde(MDA)and hydrogen peroxide were lower,the activities of superoxide dismutase,peroxidase and catalase were higher. Lycoperdon perlatum Pers.could form ectomycorrhiza with poplar,which enriched the resources of poplar ectomycorrhiza fungi.[Conclusion]The inoculation of Lycoperdon perlatum Pers.could significantly promote the growth of poplar and improve its drought resistance,which was of great significance to solve the problems of poplar growth in arid areas.