PtrbZIP3 transcription factor regulates drought tolerance of Populus trichocarpa
PtrbZIP3 transcription factor regulates drought tolerance of Populus trichocarpa
Plant basic leucine zipper(bZIP)proteins participate in plant development and a variety of abiotic stress responses.However,their roles in response to abiotic stress in Populus are still unclear.In this study,we characterized a bZIP protein,PtrbZIP3,in Populus trichocarpa which belongs to the sub-family A.PtrbZIP3 expression was highly induced by treatment with NaCl,PEG6000,or abscisic acid(ABA).PtrbZIP3 is a nuclear protein with transcriptional activation activity.Yeast one hybrid and electrophoretic mobility shift analysis showed that PtrbZIP3 can specifically bind to abscisic acid-responsive element(ABRE)to regulate the expression of ABA-dependent genes and increase the amount of ABA in P.trichocarpa overexpressing PtrbZIP3.Under PEG-simulated drought conditions,overexpression of PtrbZIP3 increases the activities of antioxidant enzymes,scavenging of reactive oxygen species,maintains cell membrane integrity,reduces stomatal apertures and water loss,and increases proline accumulation.Thus PtrbZIP3 is a transcriptional activator that plays a role in an ABA-dependent pathway to improve osmotic tolerance in P.trichocarpa.
Meiqi Zhou;Hao Cheng;Vincent L.Chiang;Wei Li;Chuanping Yang;Chao Wang;
State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;
ABRE-motif drought tolerance gene expression regulation PtrbZIP3.
2619-2619 / 1
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