杨树转录因子PdbCRF5调控盐胁迫的分子机理研究
Molecular Mechanism of Poplar Transcription Factor PdbCRF5 Regulating Salt Stress
[目的]明确PdbCRF5 基因在参与山新杨盐胁迫过程中发挥的功能及其调控机制,为通过林木基因工程育种改善植物的耐盐能力提供了理论依据和基因资源。[方法]前期对山新杨盐胁迫转录组分析发现,山新杨PdbCR F5 基因的表达受盐胁迫诱导明显,表明其可能参与山新杨盐胁迫调控。通过培育过表达和抑制表达 PdbCRF5 基因的转基因山新杨植株,我们发现 PdbCRF5 基因负调控植物对盐胁迫的耐受性。随后我们对PdbCRF5 调控的下游基因及其互作蛋白进…查看全部>>
[Objective]Clarifying the function and regulatory mechanism of the PdbCRF5 gene in participating in the salt stress process of Populus davidiana×P.bolleana provides theoretical basis and genetic resources for improving plant salt tolerance through tree genetic engineering breeding.[Method]Early analysis of the salt stress transcriptome of Populus davidiana×P.bolleana found that the expression of PdbCRF5 gene was significantly induced by salt stress,indicatin…查看全部>>
雷晓锦;房家茹;李正阳;张紫谦;刘中原;高彩球;
PdbCRF5 盐胁迫 酵母双杂交 酵母单杂交 BiFC 山新杨
PdbCRF5 Salt stress Yeast two-hybrid Yeast one-hybrid BiFC Populus davidiana×P.bolleana
2624-2625 / 2
评论