Combined Multi-omics and GLK Gene Editing Offer Insights into Coloration in Golden-leaf Tree
Combined Multi-omics and GLK Gene Editing Offer Insights into Coloration in Golden-leaf Tree
Koelreuteria paniculata is an important tree species with ornamental,medicinal,and ecological values,it is widely distributed in East Asia and introduced to Europe and North America.K.paniculata'Jinye'is a mutant variety that has a golden-color leaf phenotype.Although similar leaf color variants occur in plants,little is known of the underlying mechanism.We performed physiological,anatomical,microRNA sequencing,transcriptomic and metabolomic analyses of the golden leaf variation in the mutant,and used gene editing technology verify the function of candidate genes.Compared with wild type,the golden leaf mutant exhibited 76.05%and 44.32%decreased chlorophyll a(Chl a)and chlorophyll b(Chl b)contents,respectively,and significantly increased carotenoid content.Analysis of leaf ultrastructure revealed an abnormal chloroplast morphology and fewer lamellae in the mutant.Fifty-nine differentially expressed genes(DEGs),forty transcription factors(TFs)and forty-nine differentially expressed miRNAs(DEmiRs)involved in pigment metabolism,chloroplast development and photosynthesis,were identified.GLK and petC genes were downregulated and are involved in chloroplast development and chlorophyll synthesis,respectively.Further,the function of candidate gene GLK was validated or identified through gene editing technology in Populus tomentosa.The upregulation of PSY and PDS genes,and the downregulation of NCED gene lead to accumulation of carotenoid in mutant leaf.Varieties of chalcones and flavonols were upregulated in the mutant.Consequently,the carotenoid to chlorophyll ratio increased by more than 75%,and the accumulation of chalcones and flavonols all together lead to the golden leaf phenotype in the mutant of K.paniculata.
Ruqian Wu;Ting Guo;Xiong Yang;Sai Huang;Deyu Miao;Tingting Chen;Yinxuan Xue;Juan Li;Kai Gao;Xiaoyu Yang;Bin Guo;Xinmin An;
State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;Shanxi Academy of Forestry and Grassland Sciences,Taiyuan 030012,China;State Key Laboratory of Tree Genetics and Breeding,National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;
Koelreuteria paniculata leaf color variation chloroplast combined omics gene editing
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