榧树小孢子叶球快速伸长和开裂的分子调控
Molecular regulation of Torreya grandis staminate strobilus rapid elongation and dehiscence
[目的]挖掘榧树小孢子叶球发育过程中的快速伸长以及最终开裂的分子机制。[方法]在 2月-4 月每 7 天采集一次样品直至开裂,共采集 8 个时期的样品。对 8 个时期的小孢子叶球样品分别进行形态测量和转录组测序。通过WGCNA分解将转录组数据与形态特征相结合挖掘关键的模块和基因,接着通过榧树小孢子叶球的瞬时表达验证关键基因的功能,然后基于WGCNA相关性分析,并结合双荧光素酶试验、酵母单杂交试验等验证关键基因的调控。[结果](1)通过对榧树小孢子…查看全部>>
[Objective]Exploring the molecular mechanisms of rapid elongation and final dehiscence during the development of staminate strobilus in Torreya grandis.[Method]From February to April,we collected samples every 7 days for a total of 8 periods.Staminate strobilus samples at eight stages underwent morphological measurement and transcriptome sequencing.Key modules and genes are extracted by WGCNA analysis by fusing transcriptome data with morphological traits.Th…查看全部>>
陈伟杰;蒋宝凤;曾浩;刘智慧;陈文超;郑杉;娄和强;吴家胜;
T.grandis staminate strobilus rapid elongation dehiscence PIF4
3324-3325 / 2
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