代谢组与转录组联合分析揭示山核桃中褪黑素合成调控新机制
Metabolite profiling and transcriptome analyses reveal novel regulatory mechanisms of melatonin biosynthesis in hickory
[目的]解析山核桃中褪黑素合成通路,探究其合成调控分子机制。[方法]以山核桃三个不同发育时期的种仁为实验材料进行转录组与代谢组测定。生信分析挖掘山核桃中褪黑素合成代谢通路。通过进化树分析,蛋白同源性比对以及烟草亚细胞定位实验验证候选基因功能。进一步相关性分析挖掘褪黑素合成基因调控因子,使用双荧光素酶系统以及酵母单杂交系统对转录因子进行功能验证。[结果]代谢组数据表明山核桃中存在褪黑素及其合成代谢中间产物:色氨酸,色胺,5-羟色氨酸,血清,N-乙酰…查看全部>>
[Objective]To analyze the synthesis pathway of melatonin in hickory and explore the regulatory mechanisms.[Method]The seed kernel of three different developmental periods of hickory was used for transcriptome and metabolome determination.The melatonin synthesis pathway was determined by bioinformatics analysis.The function of candidate genes were verified by evolutionary tree,protein homology alignment,and tobacco subcellular localization experiments.The tra…查看全部>>
陈文超;张佳琦;郑杉;王占旗;徐川梅;张启香;吴家胜;娄和强;
hickory melatonin synthesis pathway new regulation mechanism
3331-3332 / 2
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